Molecular typing of Legionella pneumophila serogroup 1 isolates from patients and the nosocomial environment by arbitrarily primed PCR and pulsed-field gel electrophoresis.
Identifieur interne : 000257 ( France/Analysis ); précédent : 000256; suivant : 000258Molecular typing of Legionella pneumophila serogroup 1 isolates from patients and the nosocomial environment by arbitrarily primed PCR and pulsed-field gel electrophoresis.
Auteurs : Christine Lawrence ; Esthel Ronco ; Sylvie Dubrou ; Roland Leclercq [France] ; Charles Nauciel ; Peggy Matsiota-BernardSource :
- Journal of medical microbiology [ 0022-2615 ] ; 1999.
Descripteurs français
- KwdFr :
- ADN bactérien (analyse), Amorces ADN (), Bronches (microbiologie), Flambées de maladies (), Humains, Infection croisée (), Infection croisée (microbiologie), Infection croisée (épidémiologie), Legionella pneumophila (), Legionella pneumophila (génétique), Liquide de lavage bronchoalvéolaire (microbiologie), Maladie des légionnaires (), Maladie des légionnaires (microbiologie), Maladie des légionnaires (épidémiologie), Microbiologie de l'environnement, Polymorphisme génétique, Réaction de polymérisation en chaîne, Sérotypage, Électrophorèse en champ pulsé.
- MESH :
- analyse : ADN bactérien.
- génétique : Legionella pneumophila.
- microbiologie : Bronches, Infection croisée, Liquide de lavage bronchoalvéolaire, Maladie des légionnaires.
- épidémiologie : Infection croisée, Maladie des légionnaires.
- Amorces ADN, Flambées de maladies, Humains, Infection croisée, Legionella pneumophila, Maladie des légionnaires, Microbiologie de l'environnement, Polymorphisme génétique, Réaction de polymérisation en chaîne, Sérotypage, Électrophorèse en champ pulsé.
English descriptors
- KwdEn :
- Bronchi (microbiology), Bronchoalveolar Lavage Fluid (microbiology), Cross Infection (epidemiology), Cross Infection (microbiology), Cross Infection (prevention & control), DNA Primers (chemistry), DNA, Bacterial (analysis), Disease Outbreaks (prevention & control), Electrophoresis, Gel, Pulsed-Field, Environmental Microbiology, Humans, Legionella pneumophila (classification), Legionella pneumophila (genetics), Legionnaires' Disease (epidemiology), Legionnaires' Disease (microbiology), Legionnaires' Disease (prevention & control), Polymerase Chain Reaction, Polymorphism, Genetic, Serotyping.
- MESH :
- chemical , analysis : DNA, Bacterial.
- chemical , chemistry : DNA Primers.
- classification : Legionella pneumophila.
- epidemiology : Cross Infection, Legionnaires' Disease.
- genetics : Legionella pneumophila.
- microbiology : Bronchi, Bronchoalveolar Lavage Fluid, Cross Infection, Legionnaires' Disease.
- prevention & control : Cross Infection, Disease Outbreaks, Legionnaires' Disease.
- Electrophoresis, Gel, Pulsed-Field, Environmental Microbiology, Humans, Polymerase Chain Reaction, Polymorphism, Genetic, Serotyping.
Abstract
The ubiquity of Legionella pneumophila in aquatic habitats means that epidemiological evaluation is important for the investigation and control of nosocomial outbreaks of legionellosis. Pulsed-field gel electrophoresis (PFGE) of chromosomal DNA following digestion with SfiI is considered to be one of the most discriminative methods for detecting DNA polymorphisms amongst L. pneumophila serogroup 1 (Lp1) isolates. This paper describes an arbitrarily primed PCR (AP-PCR) method with three different primers (20-mers) for detecting DNA polymorphisms of Lp1 isolates. The AP-PCR assay was compared with PFGE analysis. Both experimental methods were found to have good discriminatory power (discrimination index of 98% and 94.3%, respectively) with 27 unrelated isolates from different geographical areas collected between 1987 and 1997. Furthermore, when the AP-PCR was used in the epidemiological investigation of nosocomial cases of infection, convergent results with the three primers allowed an epidemiological link to be established between isolates from patients and their environment. The AP-PCR method, which is rapid and easy to perform, gave results at least as discriminatory as those obtained with the PFGE method and is proposed for use in the molecular typing of Lp1 outbreaks.
DOI: 10.1099/00222615-48-4-327
PubMed: 10509473
Affiliations:
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pubmed:10509473Le document en format XML
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Pulsed-field gel electrophoresis (PFGE) of chromosomal DNA following digestion with SfiI is considered to be one of the most discriminative methods for detecting DNA polymorphisms amongst L. pneumophila serogroup 1 (Lp1) isolates. This paper describes an arbitrarily primed PCR (AP-PCR) method with three different primers (20-mers) for detecting DNA polymorphisms of Lp1 isolates. The AP-PCR assay was compared with PFGE analysis. Both experimental methods were found to have good discriminatory power (discrimination index of 98% and 94.3%, respectively) with 27 unrelated isolates from different geographical areas collected between 1987 and 1997. Furthermore, when the AP-PCR was used in the epidemiological investigation of nosocomial cases of infection, convergent results with the three primers allowed an epidemiological link to be established between isolates from patients and their environment. The AP-PCR method, which is rapid and easy to perform, gave results at least as discriminatory as those obtained with the PFGE method and is proposed for use in the molecular typing of Lp1 outbreaks.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Dubrou, Sylvie" sort="Dubrou, Sylvie" uniqKey="Dubrou S" first="Sylvie" last="Dubrou">Sylvie Dubrou</name><name sortKey="Lawrence, Christine" sort="Lawrence, Christine" uniqKey="Lawrence C" first="Christine" last="Lawrence">Christine Lawrence</name><name sortKey="Matsiota Bernard, Peggy" sort="Matsiota Bernard, Peggy" uniqKey="Matsiota Bernard P" first="Peggy" last="Matsiota-Bernard">Peggy Matsiota-Bernard</name><name sortKey="Nauciel, Charles" sort="Nauciel, Charles" uniqKey="Nauciel C" first="Charles" last="Nauciel">Charles Nauciel</name><name sortKey="Ronco, Esthel" sort="Ronco, Esthel" uniqKey="Ronco E" first="Esthel" last="Ronco">Esthel Ronco</name></noCountry><country name="France"><noRegion><name sortKey="Leclercq, Roland" sort="Leclercq, Roland" uniqKey="Leclercq R" first="Roland" last="Leclercq">Roland Leclercq</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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